甘肃部分地区致羔羊腹泻大肠埃希菌的分离鉴定及耐药性分析

为调查甘肃地区大肠埃希菌对新生羔羊腹泻的致病性及其耐药性,从甘肃省8个养殖场采集新生腹泻羔羊粪便92份。采用16S rDNA扩增结合微生物鉴别培养技术对病料中的致病性大肠埃希菌进行鉴定,随机选取分离的6株菌株进行致病性试验,并采用Kirby-Bauer纸片琼脂扩散法对分离得到的菌株进行耐药性分析。结果表明,从92份病料中分离得到21株致病性大肠埃希菌,选取的6株菌均有一定的致病性,耐药性分析结果显示该地的大肠埃希菌对羊场常用的多种抗生素耐药。表明大肠埃希菌仍是引起甘肃地区新生羔羊腹泻的主要病原菌,而且耐药性严重。     

Three-dimensional electroanatomic mapping and radiofrequency catheter ablation of ventricular arrhythmia in a dog without structural heart disease

A 1.5-year-old, female-spayed mix-breed dog was presented with recurrent episodes of shaking and excessive panting attributed to drug-refractory ventricular arrhythmia (VA) characterized predominantly by incessant periods of ventricular bigeminy. The VA had a narrow QRS morphology, suggestive of an origin near the His bundle or fascicular system. Diagnostic evaluation found no structural heart disease or underlying etiology. Three-dimensional electroanatomic mapping and radiofrequency catheter ablation were pursued. Voltage mapping demonstrated normal bi-ventricular voltage (≥1.5 mV) without any fractionated or multicomponent electrograms, indicating the absence of ventricular myocardial scar. Pace mapping identified an endocardial origin of the VA at the basal anterior septum of the left ventricle, distal to the His bundle and near the left bundle branch. Two ablation lesions were delivered to this site, and a left bundle branch block was temporarily induced. The dog recovered uneventfully. One month later, the owners reported a remarkable improvement in clinical signs, and follow-up 48-h Holter monitor found complete resolution of VA.     

兽药检验实验室信息管理系统(LIMS)的构建与实施

随着兽药检验实验室的不断升级改造,越来越多的实验室建立和实施了实验室信息管理系统(LIMS)。本文通过本单位实验室信息管理系统的构建与实施,实现了业务受理、检验任务的指派、检验记录编写、标准管理、内部审核等多个功能,有效地提升了兽药检验机构实验室的信息化水平,为今后各兽药检验机构实验室提供借鉴经验。     

中美欧兽用抗菌药与细菌耐药性管理政策对比研究

通过跟踪研究欧盟、美国与我国兽用抗菌药“禁抗、限抗与减抗”等管理政策,分析中美欧遏制耐药性风险效果及管理趋向,提出综合提升遏制细菌耐药性能力,改善饲养环境,科学使用兽用抗菌药、精准施策,研发兽医专用抗菌药,发挥中国技术优势。     

国内外兽用生物制品支原体检验方法的比较分析

为探讨优化我国兽用生物制品支原体检验方法的可能性,本文从支原体检验方法、培养基种类及培养条件、培养基质量控制和检验操作方法四个方面,对中国、美国和欧盟兽用生物制品支原体检验方法进行系统比较,分析不同方法之间的差异及优劣。结果发现,与美国《联邦法规》第九卷及《欧洲药典》相比《中国兽药典》支原体检验方法有培养基种类及配方成分全面等方面的优势,但在培养基质量控制方法、检验过程控制、检验标准制定等方面还存在不足。正视这些差距与不足,提出改进和验证的方向,可为我国兽用生物制品支原体检验的优化提供参考。